刘春朝课题组

Liu Chunzhao  Ph. D. 

课题组长、研究员

中国科学院过程工程研究所获博士学位（1998年）

Email: czliu@ipe.ac.cn

研究领域

微生物发酵制备酶制剂及酶催化反应

磁性纳米材料的制备及在工业生物技术领域应用

低劣值生物质高效生物转化能源产品

发表的主要论文(近5年)

[1]Yui Liu, Chen Guo, Chunzhao Liu.   Enhancing the resolution of (R, S)-2-octanol catalyzed by magnetic   cross-linked lipase aggregates using an alternating magnetic field,   Chemical Engineering Journal, 2015, 280: 36-40.
[2]Shikai   Wang, Stiles AR, Chen Guo, Chunzhao Liu. Harvesting microalgae by   magnetic separation: a Review, Algal Research, 2015, 9: 178-185.
[3]Hu   Jianhua, Feng Wang, Chunzhao Liu. Development of an efficient process   intensification strategy for enhancing Pfu DNA polymerase production in   recombinant Escherichia coli, Bioprocess and Biosystems Engineering,   2015, 38: 651-659.
[4]Yui Liu, Chen Guo,   Chunzhao Liu. Novel magnetic cross-linked lipase aggregates for   improving the resolution of (R, S)-2-octanol, Chirality, 2015, 27:   199-204
[5]Adekunle AE, Feng Wang, Hu   Jianhua, Anzhou Ma, Chen Guo, Chunzhao Liu. Chitosan multiple addition   enhances laccase production from Trametes versicolor, Bioprocess and   Biosystems Engineering, 2015, 38: 1973-1981.
[6]Shikai Wang,   Stiles AR, Chen Guo, Chunzhao Liu. Microalgae cultivation in   photobioreactor: an overview of light characteristics. Engineering in   Life Sciences. 2014, 14: 550-559  
[7]Feng Wang, Hu JH, Chen Guo,   Chunzhao Liu, Enhanced laccase production by Trametes versicolor using   corn steep liquor as both nitrogen source and inducer. Bioresource   Technology. 2014, 166: 602-605  
[8]Yui Liu, Chen Guo, Chunzhao   Liu. Efficient kinetic resolution of (R, S) 2-octanol catalyzed by   magnetite-immobilized Yarrowia lipolytica lipase in mixed ionic liquids.   Catalysis Letters. 2014, 144: 1552-1556 
[9]Shikai Wang, Feng   Wang, Stiles AR, Chen Guo, Chunzhao Liu. Botryococcus braunii cells:   ultrasound-intensified outdoor cultivation integrated with in situ   magnetic separation. Bioresource Technology. 2014, 167: 376-382
[10]Ling   Xu, Shikai Wang, Feng Wang, Chen Guo, Chunzhao Liu. Improved biomass   and hydrocarbon productivity of Botryococcus braunii by periodic   ultrasound stimulation. BioEnergy Research. 2014,7: 986-992
[11]Hua   Zhao, Qian Li, Junrong He, Jiangyan Yu, Junpin Yang, Chunzhao Liu,   Junhua Peng, Genotypic variation of cell wall composition and its   conversion efficiency in Miscanthus sinensis, a potential biomass   feedstock crop in China. GCB Bioenergy.2014, 6: 768-776
[12]Qian   Li, Chen Guo, Chunzhao Liu. Dynamic microwave-assisted alkali   pretreatment of cornstalk to enhance hydrogen production via co-culture   fermentation of Clostridium thermocellum and Clostridium   thermosaccharolyticum. Biomass Bioenergy. 2014, 64: 220-229
[13]Yiru   Hu, Chen Guo, Ling Xu, Feng Wang, Shikai Wang, Zanmin Hu, Chunzhao Liu.   A magnetic separator for efficient microalgae harvesting. Bioresource   Technology.2014, 158: 388-391
[14]Shikai Wang, Yiru Hu, Feng Wang,   Stiles AR, Chunzhao Liu. Scale-up cultivation of Chlorella ellipsoidea   from indoor to outdoor in bubble column bioreactors. Bioresource   Technology. 2014, 156: 117-122
[15]Yiru Hu, Chen Guo, Feng Wang,   Shikai Wang, Feng Pan, Chunzhao Liu.  Improvement of microalgae   harvesting by magnetic nanocomposites coated with polyethylenimine.   Chemical Engineering Journal. 2014, 242: 341-347 
[16]Yui Liu,   Chen Guo, Chunzhao Liu. Development of a mixed solvent system for the   efficient resolution of (R, S)-2-octanol catalyzed by   magnetite-immobilized lipase. Journal of Molecular Catalysis B:   Enzymatic. 2014, 101: 23-27 
[17]Shikai Wang, Feng Wang, Yiru Hu,   Amanda R, Chen Guo, Chunzhao Liu. Magnetic Flocculant for High   Efficiency Harvesting of Microalgal Cells. ACS Applied Materials Interfaces. 2014, 6: 109-115
[18]Wang Feng, Guo Chen, Liu   Chunzhao*, Functional magnetic mesoporous nanoparticles for efficient   purification of laccase from fermentation broth in magnetically   stabilized fluidized bed, Applied Biochemistry and Biotechnology, 2013,   171: 2165–2175.
[19]Liu Ying, Guo Chen, Sun Xitong, Liu Chunzhao*,   Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic   resolution of (R,S)-2-octanol by an integrated strategy of interfacial   activation, bioimprinting and immobilization, Bioresource Technology,   2013, 142: 415-419.
[20]Hu Yiru, Wang Feng, Wang Shikai, Liu   Chunzhao*, Guo Chen*, Efficient harvesting of marine microalgae   Nannochloropsis maritima using magnetic nanoparticles, Bioresource   Technology, 2013, 138: 387-390. 
[21]Liu Rui, Liu Ying, Liu   Chunzhao*, Development of an efficient CFD-simulation method to optimize   the structure parameters of an airlift sonobioreactor, Chemical   Engineering Research and Design, 2013, 91: 211-220.
[22]Wang Feng,   Guo Chen, Liu Chunzhao*, Immobilization of Trametes versicolor cultures   for improving laccase production in bubble column reactor intensified   by sonication, Journal of Industrial Microbiology Biotechnology,   2013, 40: 141-150. 
[23]Liu Chunzhao*, Zheng Sen, Xu Ling, Wang   Feng, Guo Chen, Algal oil extraction from wet biomass of Botryococcus   braunii by 1, 2-dimethoxyethane, Applied Energy, 2013, 102: 971-974. 
[24]Wang   Feng, Ma Anzhou, Guo Chen, Zhuang Guoqing, Liu Chunzhao*,   Ultrasound-intensified laccase production from Trametes versicolor,   Ultrasonics Sonochemistry, 2013, 20: 118-124. 
[25]Guo Bin, Stiles   Amanda R., Liu Chun-Zhao, Thidiazuron enhances shoot organogenesis from   leaf explants of Saussurea involucrata Kar. et Kir. In Vitro Cellular   and Developmental Biology – Plant, 2012, 48: 609-612.
[26]Abbasi   Bilal H, Stiles Amanda R, Saxena Praveen K, Liu Chun-Zhao. Gibberellic   acid increases secondary metabolite production in Echinacea purpurea   hairy roots. Applied Biochemistry and Biotechnology, 2012, 168:   2057-2066.
[27]Wang Feng, Huang Wei, Guo Chen, Liu Chunzhao.   Functionalized magnetic mesoporous silica nanoparticles: fabrication,   laccase adsorption performance and direct laccase capture from Trametes   versicolor fermentation broth. Bioresource Technology, 2012, 126:   117-122. 
[28]Wang Feng, Guo Chen, Wei Tao, Zhang Tian, Liu   Chunzhao. Heat shock treatment improves Trametes versicolor laccase   production. Applied Biochemistry and Biotechnology. 2012, 168: 256-265.  
[29]Xu   Ling, Liu Rui, Wang Feng, Liu Chunzhao. Development of a draft-tube   airlift bioreactor for Botryococcus braunii with an optimized inner   structure using computational fluid dynamics. Bioresource Technology,   2012, 119:.300-305.
[30]Li Qian, Liu Chunzhao. Co-culture of   Clostridium thermocellum and Clostridium thermosaccharolyticum for   enhancing hydrogen production via thermophilic fermentation of cornstalk   waste. International Journal of Hydrogen Energy, 2012, 37: 10648-10654.
[31]Xu   Lin, Wang Feng, Guo Chen, Liu Chunzhao. Improved algal oil production   from Botryococcus braunii by feeding nitrate and phosphate in an airlift   bioreactor. Engineering in Life Sciences. 2012, 12: 171-177.
[32]Cheng   Xiyu, Li Qian, Liu Chunzhao. Coproduction of hydrogen and methane via   anaerobic fermentation of cornstalk waste in continuous stirred tank   reactor integrated with up-flow anaerobic sludge bed. Bioresource   Technology, 2012, 114: 327-333.
[33]Wang Feng, Hu Yiru, Guo Chen,   Huang Wei, Liu Chunzhao. Enhanced phenol degradation in coking   wastewater by immobilized laccase on magnetic mesoporous silica   nanoparticles in a magnetically stabilized fluidized bed. Bioresource   Technology, 2012, 110: 120-124.
[34]Zhao Yan, Sun Wei, Wang Ying,   Saxena Praveen K., Liu Chunzhao. Improved mass multiplication of   Rhodiola crenulata shoots using temporary immersion bioreactors with   forced ventilation. Applied Biochemistry and Biotechnology. 2012, 166:   1480-1490.
[35]Cheng Xiyu, Liu Chunzhao. Enhanced coproduction of   hydrogen and methane from cornstalks by a three-stage anaerobic   fermentation process integrated with alkaline hydrolysis. Bioresource   Technology, 2012, 104: 373-379.
[36]Liu Chunzhao, Wang Feng,   Stiles Amanda R, Guo Chen. Ionic liquids for biofuel production:   opportunities and challenges, Applied Energy, 2012, 92: 406-414.
[37]Liu   Rui, Li Wei, Sun Liyang, Liu Chunzhao. Improving root growth and   cichoric acid derivatives production in hairy root culture of Echinacea   purpurea by ultrasound treatment. Biochemical Engineering Journal. 2012,   60: 62-66.
[38]Cheng Xiyu, Liu Chunzhao. Fungal pretreatment   enhances hydrogen production via thermophilic fermentation of cornstalk.   Applied Energy, 2012, 91: 1-6.
[39]Liu R, Sun W, Liu CZ*,   Computational fluid dynamics modeling of mass transfer behavior in a   bioreactor for hairy root culture: Part I - Model development and   experimental validation, Biotechnology Progress, 2011, 27: 1661-1671.
[40]Liu   R, Sun W, Liu CZ*, Computational fluid dynamics modeling of mass   transfer behavior in a bioreactor for hairy root culture: Part II -   Analysis of ultrasound-intensified process, Biotechnology Progress,   2011, 27: 1672-1679.
[41]Xu L, Guo C, Wang F, Zheng S, Liu CZ*, A simple and rapid harvesting method for microalgae by in situ magnetic separation, Bioresource Technology, 2011, 102: 10047-10052.
[42]Kwon YJ, Wang F, Liu CZ *, Deep-bed solid state fermentation of sweet sorghum stalk to ethanol by thermotolerant Issatchenkia orientalis IPE 100, Bioresource Technology, 2011, 102: 11262-11265
[43]Kwon YJ,   Li Q, Ma AZ, Wang F, Zhuang GQ, Liu CZ*, Effect of lignocellulosic   inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis, Bioresource Technology, 2011, 102: 8099-8104. 
[44]Cheng XY, Liu CZ*, Hydrogen production via thermophilic fermentation of cornstalk by Clostridium thermocellum, Energy and Fuels, 2011, 25: 1714-1720.
[45]Li YG*, Xu L, Huang YM, Wang F, Guo C, Liu CZ*, Microalgal biodiesel in China: opportunities and challenges, Applied Energy, 2011, 88: 3432-3437.
[46]Zhao Y, Qi LW, Wang WM, Saxena PK, Liu CZ*, Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata, Journal of Pineal Research, 2011, 50: 83-88.

授权和申请发明专利（近5年）

[1]刘春朝，王锋，郭晨，一种磁性光酶复合催化剂及其制备方法，ZL201210193905.6
[2]刘春朝，刘英，一种酶催化拆分光学纯2-辛醇的方法，ZL201310173075.5 
[3]刘春朝，王仕楷，王锋，郭晨，一种超声强化微藻培养装置及培养方法，ZL201310172944.2
[4]刘春朝, 郑森，徐玲，王锋，郭晨. 一种从布朗葡萄藻湿藻体中提取藻油的方法. 中国发明专利，专利号:ZL201110029756.5
[5]刘春朝，刘瑞，刘英，成喜雨. 一种超声波低频-高频耦合式雾化生物反应装置. 中国发明专利，专利号:ZL201110052959.6
[6]刘春朝，徐玲，王锋，郭晨. 一种光强在线调控的气升式光生物反应器.中国发明专利，专利号:ZL201110289033.9
[7]刘春朝，王锋，郭晨.一种用于微藻采收的磁分离装置及方法.中国发明专利，专利号:ZL201310182465.9
[8]刘春朝，王锋，郭晨. 一种提高变色栓菌（Trametes versicolor）漆酶产量的方法.中国发明专利，专利号:ZL201310306641.5
[9]刘春朝，成喜雨，王峰．一种使用生物预处理秸秆制备氢气的方法. ZL200710178181.7. 
[10]刘春朝，成喜雨．纤维废弃物发酵产氢气和/或甲烷的方法及其装置. ZL200810246603.4.
[11]刘春朝，郭斌．压力脉动超声雾化培养装置及其培养方法. ZL200910089439.5.
[12]刘春朝, 成喜雨．一种生物、碱复合预处理秸秆纤维原料两步发酵产气的方法. ZL 200910235234.3.
[13]刘春朝, 王锋, 郭晨,安震涛.用于生化产品分离的脉冲磁性吸附与解吸装置. ZL200910242266.6.
[14]刘春朝, 徐玲, 王锋, 郭晨. 一种采用磁性介质分离、收集微藻的方法. Zl201010171903.8.
[15]刘春朝, 权永进, 王锋. 一株耐高温东方伊萨酵母、及其应用和发酵生产乙醇的方法. ZL201110171028.8.
[16]刘春朝, 郑森, 徐玲, 王锋, 郭晨. 一种从布朗葡萄藻湿藻体中提取藻油的方法，发明专利申请号：201110029756.5
[17]郭晨, 高红帅, 王峰, 刘春朝. 一种利用离子液体从微藻中提取油脂的方法. 发明专利申请号：201110006035.2
[18]刘春朝, 刘瑞, 刘英, 成喜雨. 一种超声波低频-高频耦合式雾化生物反应装置，发明专利申请号：201110052959.6
[19]刘春朝, 权永进, 王锋. 一株耐高温东方伊萨酵母、及其应用和发酵生产乙醇的方法，发明专利申请号：201110171028.8
[20]郭晨, 王峰, 刘春朝.一种小球藻磁性分离方法，发明专利申请号：201110225274.7
[21]刘春朝, 王峰, 郭晨. 一种布朗葡萄藻磁性分离方法，发明专利申请号：201110223482.3